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A) Independent Effects. Each bioage marker <t>(DNAm,</t> mtDNAcn, or TL) was entered in a separate model with the covariate set; β therefore reflects its stand-alone association with the outcome. B) Joint effects. All three markers were entered simultaneously; β estimates indicate the unique contribution of each marker after mutual adjustment. Magenta denotes statistically significant (p < 0.05) effects.
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A) Independent Effects. Each bioage marker <t>(DNAm,</t> mtDNAcn, or TL) was entered in a separate model with the covariate set; β therefore reflects its stand-alone association with the outcome. B) Joint effects. All three markers were entered simultaneously; β estimates indicate the unique contribution of each marker after mutual adjustment. Magenta denotes statistically significant (p < 0.05) effects.
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A) Independent Effects. Each bioage marker (DNAm, mtDNAcn, or TL) was entered in a separate model with the covariate set; β therefore reflects its stand-alone association with the outcome. B) Joint effects. All three markers were entered simultaneously; β estimates indicate the unique contribution of each marker after mutual adjustment. Magenta denotes statistically significant (p < 0.05) effects.

Journal: medRxiv

Article Title: Integrative effects of Telomere Length, Epigenetic Age, and Mitochondrial DNA abundance in Alzheimer’s Disease

doi: 10.1101/2025.07.16.25331683

Figure Lengend Snippet: A) Independent Effects. Each bioage marker (DNAm, mtDNAcn, or TL) was entered in a separate model with the covariate set; β therefore reflects its stand-alone association with the outcome. B) Joint effects. All three markers were entered simultaneously; β estimates indicate the unique contribution of each marker after mutual adjustment. Magenta denotes statistically significant (p < 0.05) effects.

Article Snippet: DNA methylation (DNAm) profiling was conducted on genomic DNA extracted from whole blood sample using the Illumina Infinium Human MethylationEPIC V1 BeadChip Array, following previously described protocols .

Techniques: Marker

A) Coefficient plot. Standardized regression coefficients (β, circles) with 95 % confidence intervals (bars) for every two-way interaction (mtDNAcn × TL, mtDNAcn × DNAm, DNAm × TL)and for the three-way interaction (mtDNAcn × DNAm × TL) across baseline cognition, longitudinal cognitive decline, CSF biomarkers, and MRI measures. Magenta denotes statistically significant interactions. B) mtDNAcn × TL on global cognition. Predicted baseline global-cognition z-score versus mtDNAcn, with telomere length held at –1 SD (red, “short”), 0 SD (blue, “average”), or +1 SD (green, “long”). C) mtDNAcn × DNAm on language ability. Predicted baseline language-ability z-score versus mtDNAcn, with DNAm-age acceleration fixed at –1 SD (red, “younger”), 0 SD (blue), or +1 SD (green, “older”). D) Three-way interaction on grey-matter volume. Each facet fixes telomere length at –1, 0, or +1 SD (left-to-right). Within each facet, lines depict the mtDNAcn–grey-matter-volume slope at DNAm–1 SD (red), 0 SD (blue), and +1 SD (green).

Journal: medRxiv

Article Title: Integrative effects of Telomere Length, Epigenetic Age, and Mitochondrial DNA abundance in Alzheimer’s Disease

doi: 10.1101/2025.07.16.25331683

Figure Lengend Snippet: A) Coefficient plot. Standardized regression coefficients (β, circles) with 95 % confidence intervals (bars) for every two-way interaction (mtDNAcn × TL, mtDNAcn × DNAm, DNAm × TL)and for the three-way interaction (mtDNAcn × DNAm × TL) across baseline cognition, longitudinal cognitive decline, CSF biomarkers, and MRI measures. Magenta denotes statistically significant interactions. B) mtDNAcn × TL on global cognition. Predicted baseline global-cognition z-score versus mtDNAcn, with telomere length held at –1 SD (red, “short”), 0 SD (blue, “average”), or +1 SD (green, “long”). C) mtDNAcn × DNAm on language ability. Predicted baseline language-ability z-score versus mtDNAcn, with DNAm-age acceleration fixed at –1 SD (red, “younger”), 0 SD (blue), or +1 SD (green, “older”). D) Three-way interaction on grey-matter volume. Each facet fixes telomere length at –1, 0, or +1 SD (left-to-right). Within each facet, lines depict the mtDNAcn–grey-matter-volume slope at DNAm–1 SD (red), 0 SD (blue), and +1 SD (green).

Article Snippet: DNA methylation (DNAm) profiling was conducted on genomic DNA extracted from whole blood sample using the Illumina Infinium Human MethylationEPIC V1 BeadChip Array, following previously described protocols .

Techniques: